We aim to identify sugarcane ESTs with tissue/organ specific expression or that are stress-induced for subsequent promoter isolation and characterization. We are using in silico analyses, cDNA microarrays to select candidate ESTs and RT-PCR to confirm expression specificity. The sequences of the validated ESTs will be used to design primers for further promoter identification and cloning using a genome-walking strategy. Future studies will involve the functional characterization of these promoters. This knowledge will allow us to exploit these regulatory sequences in sugarcane biotechnology.