With equipment of last generation, scientists linked to FAPESP Program for Research on Bioenergy (BIOEN) begin to decipher genome of sugar cane. Read the news Pontapé inicial (Authorship: Agência FAPESP).
Sugarcane Genes Associated with Sucrose Content
Flávia Stal Papini-Terzi, Flávia Riso Rocha, Ricardo Zorzetto Nicoliello Vêncio, Juliana Felix, Diana Branco, Alessandro Jaquiel Waclawovski, Luiz Eduardo Vieira Del Bem, Carolina Gimiliani Lembke, Maximiller Dal-Bianco Lamas Costa, Milton Yutaka Nishiyama-Jr, Renato Vicentini, Michel Vincentz, Eugênio César Ulian, Marcelo Menossi, Gláucia Mendes Souza*
|Outlier Searching Method
Background - Sucrose content is a highly desirable trait in sugarcane as the worldwide demand for cost-effective biofuels surges. Sugarcane cultivars differ in their capacity to accumulate sucrose and breeding programs routinely perform crosses to identify genotypes able to produce more sucrose. Sucrose content in the mature internodes reach around 20% of the culms dry weight. Genotypes in the progenies reflect their genetic program and may display contrasting growth, development, and physiology, all of which affect carbohydrate metabolism. Few studies have profiled gene expression related to sugarcane's sugar content. The identification of signal transduction components and transcription factors that might regulate sugar accumulation is highly desirable if we are to improve this characteristic of sugarcane plants.
Results - We have compared thirty genotypes that have different Brix (sugar) levels and identified genes differentially expressed in internodes using cDNA microarrays. These genes were compared to existing gene expression data for sugarcane plants subjected to diverse stress and hormone treatments. The comparisons revealed a strong overlap between the drought and sucrose-content datasets and a limited overlap with ABA signaling. Genes associated with sucrose content were extensively validated by qRT-PCR, which highlighted several protein kinases and transcription factors that are likely to be regulators of sucrose accumulation. The data also indicate that aquaporins, as well as lignin biosynthesis and cell wall metabolism genes, are strongly related to sucrose accumulation. Moreover, sucrose-associated genes were shown to be directly responsive to short term sucrose stimuli, confirming their role in sugar-related pathways.
Conclusion – Gene expression analysis of sugarcane progenies contrasting for sucrose content indicated a possible overlap with drought and cell wall metabolism processes and suggested signaling and transcriptional regulators to be used as molecular markers in breeding programs or as primary targets in a sugarcane improvement program based on transgenic plants.
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