With equipment of last generation, scientists linked to FAPESP Program for Research on Bioenergy (BIOEN) begin to decipher genome of sugar cane. Read the news Pontapé inicial (Authorship: Agência FAPESP).
We aim to identify sugarcane ESTs with tissue/organ specific expression or that are stress-induced for subsequent promoter isolation and characterization. We are using in silico analyses, cDNA microarrays to select candidate ESTs and RT-PCR to confirm expression specificity. The sequences of the validated ESTs will be used to design primers for further promoter identification and cloning using a genome-walking strategy. Future studies will involve the functional characterization of these promoters. This knowledge will allow us to exploit these regulatory sequences in sugarcane biotechnology.