With equipment of last generation, scientists linked to FAPESP Program for Research on Bioenergy (BIOEN) begin to decipher genome of sugar cane. Read the news Pontapé inicial (Authorship: Agência FAPESP).
Mapping of EST-markers associated with economically
important traits to obtain a sugarcane functional genetic linkage map
The incresing availability of EST (Expressed Sequence Tags) sequences provides a potentially valuable source of new DNA markers. We aim to examine the SUCEST (Sugarcane expressed Sequence Tag Project) database and to develop EST-markers associated with economically important traits. EST-derived SSRs, EST-derived SCARs or EST-derived RFLPs are being used to enhance the resolution of an existing linkage map and to identify putative functional polymorphic gene loci in a sugarcane commercial cross. The EST markers are being screened among the 200F1 of the SP80-180xSP80-4966 mapping population. The integration of EST-derived markers will improve the map and, it renders possible to consider additional fine mapping of the genome. This will provide the means for developing ‘ideal markers’ associated with key QTLs and applications in comparative genomics.